Mikemaro collected five wins from 60 starts where he was bred before being shifted to Australia.
With regular stable driver Barnes suspended, Leonard Cain was in the sulky in the first round of heats earlier in February.
Barnes was back in the bike for his second Australian effort and it all came together, scoring by over eight metres in a dominant display in the wet conditions.
The driver made the winning move of the race, getting going well before the final turn over 2138 metres and the rest of the field had no answers in the closing stages.
“He is graded really well and was going to be off the front,” Barnes said.
“He is a safe trotter by a rule while he did not show that at times in the heats.
“His best asset is usually his manners so hopefully he can bring them on Final night.”
In winning a heat last Friday, Barnes, Butler and Mikemaro claimed 16 points for the series.
In the other heat last Friday, Majestic Harry for Darrel Graham grabbed the 16 points on offer.
Majestic Harry snared 13 points the week before in the opening heats and is set to be a big player come to the Final as well.
Also from the Butler and Barnes camp, Father Christmas finished second to Majestic Harry in the other heat and picked up the 13 points available.
Heat winners in the first round, Riley Moment and Regal Appointment did not figure in the finish in their second efforts in the series.
A respected businessman and horse owner, McNeil was a pioneer of trotting races in the Sunshine State, helping lift the horse population around Brisbane as well as increasing the profile of the form of racing.
The Jim McNeil Memorial Trotting Championship has been run since 2011.
Following Friday’s second round of heats at Albion Park, a Group 3 Final will be run at the same track with $27,186 on the line.
Trotters must contest both heats to be eligible for the Final.